epi5 episomal ipsc reprogramming kit a15960 Search Results


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Thermo Fisher epi5 kit
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Thermo Fisher epi5 episomal ipsc reprogramming kit
Epi5 Episomal Ipsc Reprogramming Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Addgene inc episomal vector for p53dd
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Addgene inc a15960
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Thermo Fisher a15960
Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive <t>for</t> <t>alkaline</t> phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm <t>(AFP,</t> j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1
A15960, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a15960/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
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Corning Life Sciences 6 well plates
Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive <t>for</t> <t>alkaline</t> phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm <t>(AFP,</t> j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1
6 Well Plates, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Corning Life Sciences matrigel-matrix hesc-qualified
Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive <t>for</t> <t>alkaline</t> phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm <t>(AFP,</t> j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1
Matrigel Matrix Hesc Qualified, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Becton Dickinson human pluripotent stem cell transcription factor analysis kit
Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive <t>for</t> <t>alkaline</t> phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm <t>(AFP,</t> j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1
Human Pluripotent Stem Cell Transcription Factor Analysis Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TissUse GmbH stem cell lines identifier tissui007-a
Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive <t>for</t> <t>alkaline</t> phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm <t>(AFP,</t> j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1
Stem Cell Lines Identifier Tissui007 A, supplied by TissUse GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher 14190136 dpbs
Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive <t>for</t> <t>alkaline</t> phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm <t>(AFP,</t> j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1
14190136 Dpbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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METTLER TOLEDO sterile 0.2–2 p2000 pipette tips air filters
Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive <t>for</t> <t>alkaline</t> phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm <t>(AFP,</t> j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1
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Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive for alkaline phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm (AFP, j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1

Journal: Molecular Autism

Article Title: Increased Ca 2+ signaling in NRXN1α +/− neurons derived from ASD induced pluripotent stem cells

doi: 10.1186/s13229-019-0303-3

Figure Lengend Snippet: Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive for alkaline phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm (AFP, j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1

Article Snippet: Low passage fibroblasts were reprogrammed to iPSCs (Merck-Millipore, SCR510; Thermo Fisher Scientific, or Epi5™ Episomal iPSC Reprogramming Kit; Invitrogen, A15960) and characterized for expression of alkaline phosphatase, NANOG, OCT4, SOX2, SSEA4, TRA-1-60, TRA-1-81, TUJ1, ASM, and AFP.

Techniques: Passaging, Staining, Expressing, Marker