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Image Search Results
Journal: Molecular Autism
Article Title: Increased Ca 2+ signaling in NRXN1α +/− neurons derived from ASD induced pluripotent stem cells
doi: 10.1186/s13229-019-0303-3
Figure Lengend Snippet: Derivation and validation of iPSCs. a Fibroblast outgrowth from the skin biopsy after 12 days of culturing. b IPSC colonies were visible and ready for collection after 24 days of reprogramming and became stable after few passaging ( c ). iPSCs were characterized and were stained positive for alkaline phosphatase ( d ) and pluripotent markers OCT4, SOX2, and NANOG and surface markers SSEA4 and TRA-1-60 and TRA-1-81 ( b – g ). Spontaneous EB differentiation has shown the expression of markers for mesoderm (ASM, h ), ectoderm (TUJ1, i ), and endoderm (AFP, j ). iPSCs also showed positive expression of proliferating marker Ki67 ( k ) and ( m ) phase marker PH3 ( l ). They were also quantified at mRNA level for the expression of OCT4 ( n ) and SOX2 ( o ). All representative images all from control line 4CCX1
Article Snippet: Low passage fibroblasts were reprogrammed to iPSCs (Merck-Millipore, SCR510; Thermo Fisher Scientific, or Epi5™ Episomal iPSC Reprogramming Kit;
Techniques: Passaging, Staining, Expressing, Marker